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概要
Vascular endothelial growth factor (VEGF-121) is a naturally-occurring VEGF-A splice variant involved in embryonic vasculogenesis and angiogenesis. VEGF binds to VEGF receptor (VEGFR)-1 and VEGFR-2, and activates Raf/MEK/ERK and PI3K/AKT pathways. VEGF-121 is released as a freely diffusible protein by a variety of normal and transformed cells. It plays an important role in neurogenesis both in vitro and in vivo (Storkebaum et al.). It has neurotrophic effects on neurons of the central nervous system and promotes growth and survival of dopaminergic neurons and astrocytes. VEGF also promotes growth and survival of vascular endothelial cells, monocyte chemotaxis, and colony formation by granulocyte-macrophage progenitor cells (Ferrara et al.). VEGF-121 contains two polypeptide chains of 121 amino acids each. This product is animal component-free.
技术资料
| Document Type | 产品名称 | Catalog # | Lot # | 语言 |
|---|---|---|---|---|
| Product Information Sheet | Human Recombinant VEGF-121, ACF | 78158, 78158.1, 78158.2 | All | English |
| Safety Data Sheet | Human Recombinant VEGF-121, ACF | 78158, 78158.1, 78158.2 | All | English |
数据及文献
Data
(A) The biological activity of Human Recombinant VEGF-121, ACF was tested by the ability to promote the proliferation of HUVECs. Cell proliferation was measured after 88 hours of culture using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which cell proliferation is at 50% of maximum. The EC50 in the example above is 4.02 ng/mL. (B) Human Recombinant VEGF-121, ACF was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining.
