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概要
Vascular endothelial growth factor (VEGF-165) is a heparin-binding homodimeric glycoprotein involved in embryonic vasculogenesis and angiogenesis. VEGF binds to VEGFR-1 (R1) and VEGFR-2 (R2), and activates Raf/MEK/ERK and PI3K/AKT pathways (Ferrara et al.). It plays an important role in neurogenesis both in vitro and in vivo (Storkebaum et al.). It has neurotrophic effects on neurons of the central nervous system and promotes growth and survival of dopaminergic neurons and astrocytes. VEGF also promotes growth and survival of vascular endothelial cells, monocyte chemotaxis, and colony formation by granulocyte-macrophage progenitor cells (Ferrara et al.).
技术资料
| Document Type | 产品名称 | Catalog # | Lot # | 语言 |
|---|---|---|---|---|
| Product Information Sheet | Human Recombinant VEGF-165 | 78073, 78073.1, 78073.2 | All | English |
| Safety Data Sheet | Human Recombinant VEGF-165 | 78073, 78073.1, 78073.2 | All | English |
数据及文献
Data
(A) The biological activity of Human Recombinant VEGF-165 was tested by its ability to promote the proliferation of HUVECs. Cell proliferation was measured using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which cell proliferation is at 50% of maximum. The EC50 in the above example is 1.7 ng/mL. (B) 4 μg of Human Recombinant VEGF-165 was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Human Recombinant VEGF-165 has a predicted molecular mass of 38.2 kDa by non-reducing SDS-PAGE.
