网站首页
概要
Vascular endothelial growth factor receptor 2/kinase insert domain receptor (VEGF R2/KDR) belongs to the class III subfamily of receptor tyrosine kinases. It is expressed on endothelial cells, endothelial progenitor cells, pancreatic duct cells, retinal progenitor cells, and megakaryocytes (Youssoufian et al.). KDR binds VEGF, which results in activation of Raf/MEK/ERK and PI3K/AKT pathways, which in turn regulate vasculogenesis and angiogenesis (Ferrara et al.; Youssoufian et al.). Studies in mouse models demonstrate that activation of KDR on hepatic endothelial cells stimulates expression of WNT2 and hepatocyte growth factor, which leads to liver regeneration (Rafii et al.). Hematopoietic regeneration and thrombopoiesis after cancer treatment is impaired in the absence of KDR in endothelial cells (Hooper et al.). KDR signaling is also implicated in lung regeneration (Rafii et al.). This protein contains a C-terminus linker (IEGRMD) to an Fc tag (Human IgG1).
技术资料
| Document Type | 产品名称 | Catalog # | Lot # | 语言 |
|---|---|---|---|---|
| Product Information Sheet | Human Recombinant VEGF R2/KDR Fc | 78130 | All | English |
| Safety Data Sheet | Human Recombinant VEGF R2/KDR Fc | 78130 | All | English |
数据及文献
Data
(A) The biological activity of Human Recombinant VEGF R2/KDR Fc was tested by the ability to inhibit VEGF-dependent HUVEC proliferation. Inhibition of cell proliferation was measured using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which cell proliferation inhibition is at 50% of maximum. The EC50 in the example above is less than 0.03 μg/mL. (B) Human Recombinant VEGF R2/KDR Fc was resolved with SDS-PAGE under reducing (+) conditions and visualized by Coomassie Blue staining.
