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概要
Interferon-gamma (IFN-γ) is the only member of the type II class of interferons. It binds to a heterodimeric receptor complex, consisting of IFN-γ receptors 1 and 2, and initiates signal transduction via the JAK/STAT pathway, which culminates in the transcription and activation of many genes that control a diverse array of immunological functions (De Weerd & Nguyen; Krause et al.). IFN-γ is produced by T and NK cells, and in smaller amounts by dendritic cells and macrophages. IFN-γ release assays, which rely on T cells releasing IFN-γ when exposed to specific antigens, are used in diagnosis of diseases including tuberculosis. IFN-γ is controlled by cytokines secreted in response to infection, such as interleukin (IL) 12 and IL-18 (Schroder). IFN-γ stimulates the antimicrobial and antitumor activity of macrophages, NK cells, and neutrophils (Billiau & Matthys) by promoting the activation of microbial effector functions including production of reactive oxygen species, NO intermediates, and complement (Schroder). IFN-γ enhances MHC class I and II expression in dendritic cells and mononuclear phagocytes, as well as the production of IL-12 by dendritic cells (Schroder).
技术资料
| Document Type | 产品名称 | Catalog # | Lot # | 语言 |
|---|---|---|---|---|
| Product Information Sheet | Rat Recombinant IFN-gamma | 78114, 78114.1 | All | English |
| Safety Data Sheet | Rat Recombinant IFN-gamma | 78114, 78114.1 | All | English |
数据及文献
Data
(A) The biological activity of Rat Recombinant IFN-gamma was tested by its ability to inhibit cell growth of WEHI-279 cells. Cell proliferation was measured using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which cell proliferation is at 50% of maximum. The EC50 in the above example is less than 0.5 ng/mL. (B) 2 μg of Rat Recombinant IFN-gamma was resolved with SDS-PAGE under reducing (+) conditions and visualized by Coomassie Blue staining.
