Human IgE ELISA Kit_产品中心_北京砺达辰生物科技有限公司

ELISAs

Human IgE ELISA Kit

For detection and measurement of human immunoglobulin E

概要

技术资料

数据及文献

概要

The Human Immunoglobulin E (IgE) ELISA Kit is designed for the quantitative detection and measurement of human IgE in biological fluids such as serum, plasma, and cell culture supernatants. IgE is predominantly found in the lungs, skin, and mucous membranes and is the least common immunoglobulin in serum. IgE plays a fundamental role in allergic reactions by stimulating degranulation of eosinophils, mast cells, and basophils through interaction with the Fc epsilon Receptor I (FcεRI).

The assay is based on the sandwich ELISA method, in which samples are added to ELISA strip plates pre-coated with capture antibodies specific for the immunoglobulin. The captured immunoglobulin is detected by addition of a biotinylated detection antibody, followed by streptavidin-horseradish peroxidase (SA-HRP), which binds the biotinylated antibody. Addition of the chromogenic enzyme substrate 3,3’,5,5’ tetramethylbenzidine (TMB) results in a colored product with an intensity directly proportional to the concentration of immunoglobulin in the sample. The concentration of the immunoglobulin is determined by comparison to a serial dilution of the immunoglobulin standard analyzed in parallel.

技术资料

Document Type	产品名称	Catalog #	Lot #	语言
Product Information Sheet	Human IgE ELISA Kit	02032, 02033	All	English
Safety Data Sheet 1	Human IgE ELISA Kit	02032, 02033	All	English
Safety Data Sheet 2	Human IgE ELISA Kit	02032, 02033	All	English
Safety Data Sheet 3	Human IgE ELISA Kit	02032, 02033	All	English
Safety Data Sheet 4	Human IgE ELISA Kit	02032, 02033	All	English
Safety Data Sheet 5	Human IgE ELISA Kit	02032, 02033	All	English
Safety Data Sheet 6	Human IgE ELISA Kit	02032, 02033	All	English
Safety Data Sheet 7	Human IgE ELISA Kit	02032, 02033	All	English
Safety Data Sheet 8	Human IgE ELISA Kit	02032, 02033	All	English
Safety Data Sheet 9	Human IgE ELISA Kit	02032, 02033	All	English
Safety Data Sheet 10	Human IgE ELISA Kit	02032, 02033	All	English
Safety Data Sheet 1	Human IgE ELISA Kit	02033	All	English
Safety Data Sheet 2	Human IgE ELISA Kit	02033	All	English

数据及文献

Data

Representative Standard Curve

• Reportable Range: 0.1 - 32 ng/mL. This is the concentration range in which measurement of the analyte can be done with the highest precision, accuracy, and linearity.
• Sensitivity: The limit of detection of this assay is 0.069 ng/mL. This is the analyte concentration with absorbance two standard deviations higher than the zero standard.
• Accuracy: The analyte standard of this ELISA was calibrated against NIBSC international standard 75/502.
• Recovery: A mid-curve recovery of 80 - 86% was determined by spiking defined amounts of analyte standard into serum or plasma samples in repeated experiments.
• Precision: The intra-assay precision of this assay is 2.8% (CV). The inter-assay precision of this assay is 5.5% (CV).